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egfp appl1 δptb  (Addgene inc)


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    Addgene inc egfp appl1 δptb
    Egfp Appl1 δptb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+appl1+%CE%B4ptb/GFP-APPL1-%CE%94PTB+(Plasmid+%2359768)/pmc07889693-233-16-22
    Average 92 stars, based on 1 article reviews
    egfp appl1 δptb - by Bioz Stars, 2026-09
    92/100 stars

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    Transfection:

    Article Title: Rapid whole cell imaging reveals a calcium-APPL1-dynein nexus that regulates cohort trafficking of stimulated EGF receptors
    Article Snippet: .. Cells were transfected with pEGFPC1-human APPL1, a gift from Pietro De Camilli (Addgene plasmid #22198) , EGFP-APPL1-ΔPTB, a gift from Donna Webb (Addgene plasmid #59768) , DsRed-p150 217-548, a gift from Trina Schroer (Addgene plasmid #51146) , EEA1 TagRFP-T, a gift from Silvia Corvera (Addgene plasmid #42635) , pEGFR-PATagRFP, a gift from Vladislav Verkhusha (Addgene plasmid #31950) . .. Cells were transfected with a total of 1 μg DNA (plasmid of interest—0.2 μg, blank DNA—0.8 μg) for single protein expression or (plasmids of interest—0.2 μg + 0.2 μg, blank DNA—0.6 μg) using lipofectamine 3000 (Thermo Fisher Scientific) or the Neon electroporator (Invitrogen) at 1200 mV 20 ms, 2 pulses for RPE1 and 1005 mV 35 ms, 2 pulses for HeLa.

    Plasmid Preparation:

    Article Title: Rapid whole cell imaging reveals a calcium-APPL1-dynein nexus that regulates cohort trafficking of stimulated EGF receptors
    Article Snippet: .. Cells were transfected with pEGFPC1-human APPL1, a gift from Pietro De Camilli (Addgene plasmid #22198) , EGFP-APPL1-ΔPTB, a gift from Donna Webb (Addgene plasmid #59768) , DsRed-p150 217-548, a gift from Trina Schroer (Addgene plasmid #51146) , EEA1 TagRFP-T, a gift from Silvia Corvera (Addgene plasmid #42635) , pEGFR-PATagRFP, a gift from Vladislav Verkhusha (Addgene plasmid #31950) . .. Cells were transfected with a total of 1 μg DNA (plasmid of interest—0.2 μg, blank DNA—0.8 μg) for single protein expression or (plasmids of interest—0.2 μg + 0.2 μg, blank DNA—0.6 μg) using lipofectamine 3000 (Thermo Fisher Scientific) or the Neon electroporator (Invitrogen) at 1200 mV 20 ms, 2 pulses for RPE1 and 1005 mV 35 ms, 2 pulses for HeLa.



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    egfp appl1 δptb  (Addgene inc)
    92
    Addgene inc egfp appl1 δptb
    Egfp Appl1 δptb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+appl1+%CE%B4ptb/GFP-APPL1-%CE%94PTB+(Plasmid+%2359768)/pmc07889693-233-16-22
    Average 92 stars, based on 1 article reviews
    egfp appl1 δptb - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

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